Abstract :Immunofluorescence (IF) is a technique widely used in medical research and diagnostics due to its simplicity, accuracy, and rapidity. In this article, we present the applications of IF in the diagnosis of seven oral diseases; namely, pemphigus vulgaris, mucous membrane pemphigoid, linear IgA disease, oral lichen planus, oral mucosal lupus erythematosus, epidermolysis bullosa acquisita, and chronic ulcerative stomatitis. A brief outline of each disease, in terms of its clinical features and underlying pathophysiology (based on current understanding), is also provided so that the relevance of the IF findings may be better understood and appreciated. This review aims to serve as a ready reference for both clinicians and students alike, as well as to demonstrate how this 76-year-old technique remains relevant in the field of oral medicine today.